SELPLG Antikörper (FITC)

Produktnummer ABIN488144

Der Maus Monoklonal Anti-SELPLG Antikörper wurde für FACS validiert. Er ist geeignet, SELPLG in Proben von Human zu detektieren.

Kurzübersicht für SELPLG Antikörper (FITC) (ABIN488144)

Target: SELPLG (Selectin P Ligand (SELPLG))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SELPLG Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS)

Klon: PL2

Produktdetails anti-SELPLG Antikörper (FITC)

Spezifität: This antibody reacts with Human PSGL-1 antigen.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Protein-A Sepharose Chromatography.

Immunogen: Human Neutrophil PSGL-1. Remarks: This Hybridoma was established by fusion of mouse myeloma cell P3-X63-Ag8-653 with Balb/c mouse splenocyte.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 10-20 μg/mL (final concentration). Positive Controls: Jurkat, HL60.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Flow Cytometric Analysis for Floating CellsProtocol 1We use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Add 10 µL of normal goat serum to the cell pellet aftertapping. Mix well, and incubate for 5 minutes at roomtemperature (20~25°C). 3) Add 30 µL of the FITC labeled CD162 (PL2) monoclonal antibody (10-20 µg/mL) dilutedwith the washing buffer. Mix well, and incubate for 30 minutes at room temperature(20~25°C). 4) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 5) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Protocol 2We usually use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Resuspend the cells with PBS containing 25% normal goat serum, 1 mg/mL normalhuman IgG and 0. 1% NaN3 (5x10e6 cells/mL). 3) Add 20 µL of the non diluted FITC labeled CD162 (PL1) monoclonal antibody (50 µg/mL)into each tube. 4) Add 50 µL of the cell suspension into each tube. Mix well, and incubate for 30 minutesat room temperature (20~25°C)5) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 6) Resuspend the cells with 500 μL of the washing buffer and analyze by a flow cytometer.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.05 mg/mL

Buffer: PBS, pH 7.2 containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu SELPLG

Target: SELPLG (Selectin P Ligand (SELPLG))

Andere Bezeichnung: CD162 / PSGL1

Hintergrund: CD162 is a 120kD molecule also known as P-Selectin Glycoprotein Ligand I (PSGL-1). CD162 is the major counter receptor for P-selectin and is involved in neutrophil attachment and rolling to endothelium. In peripheral blood CD162 is expressed by monocytes, granulocytes, T cells and some B cells.Synonyms: P-selectin glycoprotein ligand 1, PSGL-1, SELPLG, Selectin P ligand

Gen-ID: 6404

NCBI Accession: NP_002997

UniProt: Q14242